Effects of inhibitory compounds derived from lignocellulosic biomass on the growth of the wild-type and evolved oleaginous yeast Rhodosporidium toruloides

The presence of toxic compounds in lignocellulosic hydrolysates is one the main problems affecting efficiency hydrolysate-based fermentation processes. Understanding effects biomass-derived inhibitors on performance microbial strain essential to develop strategies able result an improved performance. In present study, efforts were done elucidate growth oleaginous yeast Rhodosporidium toruloides. Furfural, 5-hydroxymethylfurfural (5-HMF), acetic acid, levulinic benzoic p-coumaric ferulic vanillic vanillin and syringaldehyde inhibitory investigated. Assays performed using a wild-type evolved R. For individual inhibitors, more than 3 mM acid completely inhibited both strains, while less toxic, presenting IC50 values 16 15 mM, respectively. subsequent step, assays media containing different combinations Placket-Burman experimental design was used identify most severe mixture, as well combination growth. Results revealed that furfural potent inhibitor toruloides, followed by 5-HMF. furfural, 5-HMF, vanillin, resulted inhibition this yeast. showed ability tolerate compounds, suggesting adaptive laboratory evolution potential strategy obtain yeasts with grow biomass hydrolysates.